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The day before the assay, frozen aliquots of the above-mentioned BHK cell lines were thawed, washed once in PBS (Gibco, #14190-094) and seeded in 40 L medium in a white 384-well culture plate (PerkinElmer, #6007688) at a cell density of 4000 cells/well and incubated in plastic bags over night at 37 C and 5% CO 2

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Some must be injected because they will not survive the digestive system intact if swallowed

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This work was supported by VA-merit BX002086 (to P.D.), DK088902 (to A.B.S.) and BX002761 (to A.B.S.)