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Figure 1: Chemical structure of Glutathione [source: Accessed: 10 Oct 2012] The presence of the reactive sulphydryl group (originating from the cysteine residue) gives rise to GSHs nucleophilic nature and so it reacts readily with electrophiles which can present high risk of toxicity to the cell by causing oxidative damage such as lipid peroxidation to cell membranes, and interfering with the activity of essential proteins and DNA synthesis
It does exactly what I need it to do
Growth media was replaced with binding buffer [phenol-free DMEM containing 25 mM HEPES and 0.1% (w/v) BSA] containing 0.7 nM 125 I-Ex(939) or 0.15 nM 125 I-hGLP-1 and increasing concentrations of unlabelled ligand
Avoid high calorie fatty food and too much spice in your diet