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Synthetic buffer A solution of 4 g/L of L-( +)-tartaric acid, 3 mg of iron/L, in the form of iron (III) chloride hexahydrate, and 0.3 mg of copper/L in the form of copper (II) sulfate pentahydrate adjusted to pH 3.5 with sodium hydroxide was used for all experiments
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The samples were incubated with mouse primary monoclonal antibody, anti-Nestin 1:200 (Santa Cruz Biotechnology, Inc., Dallas, TX, USA), anti-GAP43 (1:500
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