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Hybridization buffer (pH 7.4) contained 10 mM Tris-HCl, 50 mM NaCl, 1 mM EDTA and 10 mM MgCl 2

A 5 L aliquot of the resulting worm lysate was used as the DNA template for PCR to confirm genetic background

Levels of glutathione decrease as we age, glutathione is the bodys most powerful antioxidant protecting us from environmental toxins and pollutants

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